Journal: mBio

Article Title: The Neutrally Charged Diarylurea Compound PQ401 Kills Antibiotic-Resistant and Antibiotic-Tolerant Staphylococcus aureus

doi: 10.1128/mBio.01140-20

Figure Lengend Snippet: The insulin-like growth factor receptor (IGF-1R) inhibitor PQ401 exhibits bactericidal activity against S. aureus strain MW2 with no detectable resistance development. (A) Chemical structure of PQ401. (B) MRSA-infected C. elegans was treated with 5 μg/ml PQ401, 10 μg/ml vancomycin (Van, positive control), and 0.1% DMSO (DMSO, negative control). Dead worms were stained with SYTOX Orange. (C) Exponential-phase S. aureus MW2 was treated with PQ401 or vancomycin for 4 h. The bacterial viability was measured at hourly intervals. The limitation of detection is 2 × 10 2 CFU/ml. Error bars denote SD ( n = 3). (D) Three attempts to develop MRSA resistance to PQ401 (SP 1, 2, and 3) and to ciprofloxacin (Cipro) over 25 days.

Article Snippet: Methicillin-susceptible S. aureus strain Newman ( ); methicillin-resistant S. aureus (MRSA) strains MW2 BAA-1707 ( ) ATCC 33591, and JE2 ( ); vancomycin-resistant S. aureus strain VRS1 ( ); 11 clinical S. aureus isolates ( ); Enterococcus faecium E007 ( , ); vancomycin-resistant E. faecium strain C68 ( , ); Klebsiella pneumoniae WGLW2 (BEI Resources, Manassas, VA, USA); Acinetobacter baumannii ATCC 17978 ( ); Pseudomonas aeruginosa PA14 ( ); and Enterobacter aerogenes ATCC 13048 were used to test antimicrobial activity ( to ).

Techniques: Activity Assay, Infection, Positive Control, Negative Control, Staining

Journal: mBio

Article Title: The Neutrally Charged Diarylurea Compound PQ401 Kills Antibiotic-Resistant and Antibiotic-Tolerant Staphylococcus aureus

doi: 10.1128/mBio.01140-20

Figure Lengend Snippet: MIC of PQ401 against MRSA strains

Article Snippet: Methicillin-susceptible S. aureus strain Newman ( ); methicillin-resistant S. aureus (MRSA) strains MW2 BAA-1707 ( ) ATCC 33591, and JE2 ( ); vancomycin-resistant S. aureus strain VRS1 ( ); 11 clinical S. aureus isolates ( ); Enterococcus faecium E007 ( , ); vancomycin-resistant E. faecium strain C68 ( , ); Klebsiella pneumoniae WGLW2 (BEI Resources, Manassas, VA, USA); Acinetobacter baumannii ATCC 17978 ( ); Pseudomonas aeruginosa PA14 ( ); and Enterobacter aerogenes ATCC 13048 were used to test antimicrobial activity ( to ).

Techniques:

Journal: mBio

Article Title: The Neutrally Charged Diarylurea Compound PQ401 Kills Antibiotic-Resistant and Antibiotic-Tolerant Staphylococcus aureus

doi: 10.1128/mBio.01140-20

Figure Lengend Snippet: Minimum bactericidal concentration of PQ401 against MRSA strains

Article Snippet: Methicillin-susceptible S. aureus strain Newman ( ); methicillin-resistant S. aureus (MRSA) strains MW2 BAA-1707 ( ) ATCC 33591, and JE2 ( ); vancomycin-resistant S. aureus strain VRS1 ( ); 11 clinical S. aureus isolates ( ); Enterococcus faecium E007 ( , ); vancomycin-resistant E. faecium strain C68 ( , ); Klebsiella pneumoniae WGLW2 (BEI Resources, Manassas, VA, USA); Acinetobacter baumannii ATCC 17978 ( ); Pseudomonas aeruginosa PA14 ( ); and Enterobacter aerogenes ATCC 13048 were used to test antimicrobial activity ( to ).

Techniques: Concentration Assay

Journal: mBio

Article Title: The Neutrally Charged Diarylurea Compound PQ401 Kills Antibiotic-Resistant and Antibiotic-Tolerant Staphylococcus aureus

doi: 10.1128/mBio.01140-20

Figure Lengend Snippet: Proportion of each ionized form of PQ401 and their MICs against MRSA strain MW2 ^a

Article Snippet: Methicillin-susceptible S. aureus strain Newman ( ); methicillin-resistant S. aureus (MRSA) strains MW2 BAA-1707 ( ) ATCC 33591, and JE2 ( ); vancomycin-resistant S. aureus strain VRS1 ( ); 11 clinical S. aureus isolates ( ); Enterococcus faecium E007 ( , ); vancomycin-resistant E. faecium strain C68 ( , ); Klebsiella pneumoniae WGLW2 (BEI Resources, Manassas, VA, USA); Acinetobacter baumannii ATCC 17978 ( ); Pseudomonas aeruginosa PA14 ( ); and Enterobacter aerogenes ATCC 13048 were used to test antimicrobial activity ( to ).

Techniques:

Journal: mBio

Article Title: The Neutrally Charged Diarylurea Compound PQ401 Kills Antibiotic-Resistant and Antibiotic-Tolerant Staphylococcus aureus

doi: 10.1128/mBio.01140-20

Figure Lengend Snippet: PQ401 has bactericidal potency and shows synergism with gentamicin against antibiotic-tolerant MRSA. Antibiotic-tolerant cells of MRSA MW2 were treated with 100× MIC vancomycin (Van), gentamicin (Gm), or ciprofloxacin (Cipro) (A); the indicated concentrations of PQ401 (B); or 10 μg/ml gentamicin (Gm) in combination with various concentrations of PQ401 for 4 h (C). CFU counts of viable cells were measured by serial dilution and plating on TSA plates. Results are shown as means ± SD ( n = 3).

Article Snippet: Methicillin-susceptible S. aureus strain Newman ( ); methicillin-resistant S. aureus (MRSA) strains MW2 BAA-1707 ( ) ATCC 33591, and JE2 ( ); vancomycin-resistant S. aureus strain VRS1 ( ); 11 clinical S. aureus isolates ( ); Enterococcus faecium E007 ( , ); vancomycin-resistant E. faecium strain C68 ( , ); Klebsiella pneumoniae WGLW2 (BEI Resources, Manassas, VA, USA); Acinetobacter baumannii ATCC 17978 ( ); Pseudomonas aeruginosa PA14 ( ); and Enterobacter aerogenes ATCC 13048 were used to test antimicrobial activity ( to ).

Techniques: Serial Dilution

Journal: The Malaysian Journal of Medical Sciences : MJMS

Article Title: Antibacterial Effects of Various Types of Bee Products in Malaysia: A Systematic Review

doi: 10.21315/mjms2024.31.3.3

Figure Lengend Snippet: Overview characteristics of included studies

Article Snippet: Ng and Lim ( ) , Gelam honey , Staphylococcus aureus methicillin sensitive (MSSA) strains (ATCC 25923 and ATCC 6538) and methicillin-resistant (MRSA) strains (ATCC 33592 and ATCC 33591) , Agar well diffusion method , Zone of inhibition against tested bacteria MSSA ATCC 25923: 0.0–10.7 mm MSSA ATCC 6538: 0.0–10.0 mm MRSA ATCC 33592: 0.0–13.0 mm MRSA ATCC 33591: 0.0–13.7 mm.

Techniques: Bacteria, Diffusion-based Assay, Inhibition, Concentration Assay, Biofilm Production Assay, Microscopy, Disruption, Confocal Laser Scanning Microscopy, Imaging, Membrane, Northern Blot